Bombesin-saporin

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August undefined, 2024

WebJun 20, 2012 · Twenty-four hours after transfection, 20 μg/ml of NMB-saporin or blank-saporin or 2.5 μg/ml of bombesin-saporin were added to cultured media and 48 h later … WebOct 18, 2024 · The neurotoxin bombesin–saporin (BB-sap) binds to and is internalized by GRPR neurons; however, it is not internalized by those expressing the related receptor …

Bombesin Medical Definition Merriam-Webster Medical

WebNMB-saporin (NMB-sap) results in efficient ablation while bombesin-saporin (Bomb-sap) or blank-saporin (blank-sap) have no effect on NMBR-expressing cells. Cells transfected … WebAug 21, 2013 · ociated itch in mice. Transdermal inoculation of human herpes virus 1 on the midflank produced herpes zoster-like skin lesions and caused the mice to scratch (itch … harley catalog parts

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WebSep 1, 2024 · Bombesin-saporin treatment. The mice were given a single i.t. injection of bombesin-saporin (400 ng/mouse) or blank-saporin (400 ng/mouse). These mice were … WebDec 17, 2024 · Bombesin-saporin or blank-saporin was injected 3 days before DCP modeling, and behavioral or immunohistological experiments were performed 24 days later. Open Field Test. Mice were placed in the center of an open box (40 cm 3 × 40 cm 3 × 40 cm 3) and video-taped individually. The animal movement was tracked by an overhead … WebFeb 14, 2012 · We found that after stimulation with both histamine and chloroquine, fewer Fos‐positive cells were present in mice treated with bombesin–saporin compared with those treated with saporin alone. The reduction in Fos expression was greater with chloroquine than with histamine, and the distribution of Fos‐positive cells was also different. changing vertical axis values in excel chart

A neuropeptide code for itch Nature Reviews Neuroscience

A central role for spinal dorsal horn neurons that express …

WebOct 30, 2015 · Although intrathecal injection of a GRPR antagonist or ablation of GRPR-expressing neurons with conjugated bombesin-saporin (fig. S6) was effective in blocking chloroquine-evoked itch (Fig. 3, D and F), these manipulations failed to blunt scratching in response to mechanical stimulation in NPY::Cre IN–ablated mice (Fig. 3, C and E). ... WebNational Center for Biotechnology Information harley cb microphoneWebJan 2, 2024 · Bombesin-saporin was injected 3 weeks after viral injection. And the behavioral and histological experiments were performed 10 days after bombesin-saporin or blank-saporin injection. Tamoxifen induction. For labeling the GRPR-positive neurons with fluorescence, we have crossed GRPR-iCreERT2 and Ai9 mice. To induce the Cre … harley catch can

"WebFeb 14, 2012 · We found that after stimulation with both histamine and chloroquine, fewer Fos‐positive cells were present in mice treated with bombesin–saporin compared with … " - Bombesin-saporin

Bombesin-saporin

National Center for Biotechnology Information

WebMice were given a single intrathecal injection of Bombesin-saporin (400 ng). Intrathal OT-induced scratching bouts was then recorded for 30 min two weeks after Bombesin … WebMice were given a single intrathecal injection of blank-saporin (400 ng/5 µl), bombesin-saporin (200, 300, 400 ng/5 µl) or saline (5 µl). Mice were used for behavior experiments at 2 weeks after the treatment. Some mice were perfused at 2 or 4 weeks after treatment for molecular marker analysis. Itch behavior

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WebNational Center for Biotechnology Information Webnoun. bom· be· sin ˈbäm-bə-sin. : a polypeptide that is found in the brain and gastrointestinal tract and has been shown experimentally to cause the secretion of various substances …

WebNov 1, 2014 · Intrathecal administration of the neurotoxin bombesin-saporin reduces or abolishes pruritogen-evoked scratching behavior. We investigated whether spinal neurons that respond to intradermal (ID) injection of pruritogens also respond to spinal superfusion of bombesin and vice versa. Single-unit recordings were made from superficial lumbar … WebJun 20, 2012 · 0.13 µg of stable SP-saporin (SSP-saporin) or 1.3 µg of bombesin-saporin or 10 µg of blank- saporin (Advanced Targeting System, San Diego in 20µl of PBS) were administered to the lumbar 4 to 5 ...

WebFeb 8, 2016 · Bilateral preBötC injections of saporin conjugated with either bombesin (BBN–SAP; Advanced Targeting System; 200 nl, 6.2 ng) or a non-targeted peptide … WebBombesin-SAP [IT-40, KIT-40] a tool for eliminating cells that express gastrin releasing peptide (GRP) receptor; targeted via bombesin, eliminated via saporin. Bombesin is a … Anti-DAT-SAP is a chemical conjugate of a rat monoclonal antibody to the second …

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Web2.2. Saporin injection To neurotoxically ablate NK1R- or GRPR-expressing spinal neurons, OVA-treatedmice received anintrathecal injection of either blank saporin (SAP, 400 ng/5mL, Advanced Targeting Systems, San Diego, CA), substance P-SAP (SP-SAP, 100 ng/5 mL, Advanced Targeting Systems), or bombesin-SAP (BB-SAP, 400 ng/5 mL, Advanced ... harley caudle attorneyWebMay 31, 2024 · Bombesin-saporin (Catalog: IT-40) and blank-saporin (Catalog: IT-21) were purchased from Advanced targeting systems. Animals. Oprm1. fl/fl (stock No: 030074), Vgat-ires-Cre (stock No: 016962), Sst-ires-Cre (stock No:013044), Trpv1-Cre (stock No:017769), Ai32 (stock No:024109), C57BL/6J (stock No:000664), NOD. CB- harley ccaWebNov 1, 2014 · Abstract. Intrathecal administration of the neurotoxin bombesin-saporin reduces or abolishes pruritogen-evoked scratching behavior. We investigated whether … harley cc