WebNov 4, 2015 · Cycloheximide (Naramycin A) 是一种抗真菌 (antifungal) 抗生素,是真核生物蛋白质合成 (protein synthesis) 的抑制剂,抑制体内蛋白质合成和 RNA 合成的 IC50 值分别为 532.5 nM 和 2880 nM。Cycloheximide 可抑制铁死亡 (ferroptosis) 并抑制自噬 (autophagy)。- 高纯度,全球文献引用。 WebJan 1, 2015 · 100 mg/ml cycloheximide (CHX) stock solution (Sigma-Aldrich, catalog number: C7698) ... For an unfamiliar protein, it is recommended to start with a 4-hour interval and chase till 24 h. Cycloheximide may cause cytotoxicity to certain cells if cells …
How do I determine protein half life with cycloheximide or cell …
WebMar 26, 2024 · How to open CHX files. Important: Different programs may use files with the CHX file extension for different purposes, so unless you are sure which format your CHX file is, you may need to try a few different programs. While we have not verified the apps … WebMay 19, 2024 · 这个实验只需要用到一个叫放线菌酮(chx)的试剂,就能简单的解释一下为什么蛋白表达有差异。 当然,你非要给我说什么蛋白质组半衰期动力学这种尖端技术,不好意思,玩儿不起。 brantley gilbert playlist youtube
Cycloheximide - Wikipedia
WebPulse-chase and cycloheximide (CHX)-chase assays. (a) Pulse-chase assay. Pulse-chase analysis involves labelling of all proteins by culturing cells in the ‘pulse phase’ medium containing a radioactive labeled amino acid and subsequently following the proteins in ‘chase phase’ medium containing the non-radioactive labeled form of the amino acid.; … WebNov 11, 2024 · 五步法:快速测定蛋白质稳定性. 细胞中的蛋白质通过合成和降解等活动控制处于恒定状态。. 为了使细胞更高效,减少不必要的蛋白质负荷,细胞中的大多数蛋白质都有一个明确定义的半衰期。. 细胞进化到降解蛋白质的另一个原因是,确保及时清除已经完 … WebCycloheximide is supplied as a lyophilized powder. For a 10 mg/ml stock, carefully weigh out and reconstitute 50 mg in 5 ml DMSO or EtOH. Working concentrations and length of treatments vary depending on the desired … brantley gilbert newnan ga