Ctd110.6 antibody
WebJul 23, 2024 · O-GlcNAc Antibody (CTD110.6) is a mouse monoclonal IgM κ O-GlcNAc antibody, cited in 62 publications, provided at 200 µg/ml; raised against O-GlcNAc … WebCTD110.6, 18B10 and 9D1 are anti-O-GlcNAc monoclonal antibodies. B. HEK 293T cells were treated with DMSO, 50 μM TG or 50 μM 5SG for 24 hours, 200 nM rapamycin …
Ctd110.6 antibody
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WebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. WebApr 19, 2011 · The O-GlcNAc specific antibody, RL-2, is not N-GlcNAc 2-modifed proteins , so RL-2 antibodies may be more specific for O-GlcNAc-modifed proteins than …
WebResults: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized. Webat 6 h after the treatment, i.e. at the same time that an increase was previously noted for proteins identified by their reaction with CTD110.6 antibodies (Figure 1b). The immunoprecipitation analysis showed that CTD110.6 antibodies reacted with all four of the lower molecular weight forms that were immunoprecipitat-
WebClone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). WebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 …
WebImmunofluorescence staining of CTD110.6 on methanol fixed HeLa cells at 10 µg/ml. Following fixation, the cells were incubated with the primary antibody for one hour at … how busy is disneyland tomorrowWebNov 22, 2024 · Results indicate Cdcp1 (CD318) as a ligand of T-cell differentiation antigen CD6 (CD6) and a potential target for the diagnosis and treatment of … how busy is disneyland paris in aprilWebFigure 6 HRECs exposed to TNF-alpha, and high glucose for 24 h demonstrated alterations in VE-Cadherin and defective complex formation. The whole cell lysates were immunoprecipitated with indicated antibodies, and the immunocomplexes were analyzed for O-GlcNAcylation using anti-O-GlcNAc antibody (clone CTD110.6) ( A ). how many pairs of animals were on the arkWebThe antibody CTD110.6 is often used to detect changes in the O-GlcNAc modification. Recently, it has been demonstrated that CTD110.6 recognizes N-linked N,N'-diacetylchitobiose, which is thought ... how busy is disney world during spring breakhttp://bb-china.net/search.html?brand=42&pcategory=0&classd=0&use=0&kw=&promotions=2&inventory=2&category=17&page=21 how busy is disney world in julyWebApr 24, 2024 · Among these antibodies, CTD110.6 and RL2 are the most widely used. However, due to a certain degree of amino acid sequence dependency, these antibodies cannot detect all proteins with O-GlcNAcylation. Additionally, a few antibodies for site-specific protein O-GlcNAcylation have been reported, ... how busy is disneyland paris in marchWebTarget Information. CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b … how busy is disney thanksgiving week