Hct116 p53+/+ cells

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WebHNK affects stem cell marker expression. (A) HCT116 cells were grown in spheroid growth media in low adherent plates and treated with 25 µM HNK. After 5 days, the colonospheres were photographed and counted. ... Lysates from HNK treated cells show an increase in p53 phosphorylation and PUMA expression. (C) Cytoplasmic extracts from HNK treated ... WebMay 29, 2024 · HCT116 p53 −/− cells were plated in 96-well plates so they will be at 70–90% confluence at the time of transfection. DNA-lipid complexes were prepared in 10 μL/well Opti-MEM reduced serum medium (Gibco), using 0.1 μg/well plasmid DNA and 0.2 μL/well of P3000 and Lipofectamine 3000 reagents (Thermo Fischer, Germany). ...

Regulation of p53 stability and function in HCT116 colon …

WebJul 15, 2024 · Indeed, a low dose of Olaparib markedly activated the p53 pathway in RMRP-knockout HCT116 p53+/+ cells, whereas having a marginal effect on RMRP-proficient colorectal cancer cells (Fig. 7J), which was also verified by the expression of p53 target genes (Fig. 7K). These results strongly suggested that RMRP could confer resistance to … WebMar 20, 2024 · The expression of CDKN1A was not enhanced in solvent-treated hypoxic HCT116 p53 +/+ cells (DMSO normoxia vs hypoxia, P = 0.56 by unpaired t-test, two-tailed), corroborating the results from flow ... most expensive hotel in budapest

P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint …

WebDec 22, 2005 · The three TRAIL-sensitive cell lines (HCT116 p53 +/+, HCT116 p53 −/−, and H630) expressed a relatively high level of DR5, whereas DR4 was only detected on the cell surface of H630 cells and was expressed at a lower level than DR5. In addition, the H630 cell line was the only one found to express a TRAIL decoy receptor, DcR1, on its … WebAug 12, 2016 · Loss of p53 function in cancer cells contributes to tumor progression in the presence of fibroblasts. (A and B) Relative expression of p53 and its downstream targets were determined in HCT116 +/+ and HCT116 −/− cells using qRT–PCR (A) (n = 3/group) and western blotting (B); *P < 0.05 versus HCT116 +/+ cells.(C) HCT116 +/+ or HCT116 … WebTo further explore the inhibitory effects of PNAP-6 on colon cancer cells, the viability of HCT116 (p53 wild type) and HT-29 (p53 mutant) cells were examined by MTT assay … most expensive hotel brisbane

p53 modulates homologous recombination by transcriptional …

HCT116 cells - Wikipedia

WebHCT116 p53–/– cells as well as a derivative cell line expressing the p53-Luc fusion were tested and the data presented are an average of three independent experiments. Activation of the p53 protein protects the organism against the propagation of cells that carry damaged DNA with potentially transforming mutations. MDM2, a p53-specific E3 ... WebJul 20, 2024 · Isogenic human colorectal cancer cell lines HCT116 (p53 +/+ and p53-/-) were irradiated with high-LET carbon ions. Cell survival was determined by the standard … mini beard straightenerWebJun 10, 2016 · Three biological replicates were used for p value, p*<0.01. (H,I) NGFR mRNA expression is inhibited by p53 knockdown. HCT116 p53+/+ (H) and H460 (I) cells were transfected with p53 or control siRNA for 72 hr, and Doxorubicin or 5-Fluorouracil was supplemented 15 hr before the cells were harvested for q-PCR (Mean ± SEM, n = 3). … minibear instant camera review

"WebDownload scientific diagram Influence of PHA680632 on cell cycle in p53wt vs p53−/− HCT116 cells. (A and B) analysis of the cell cycle. (A) Quantitative data of cell cycle … " - Hct116 p53+/+ cells

Hct116 p53+/+ cells

Regulation of p53 Stability and Function in HCT116 Colon Cancer …

WebDec 2, 2005 · The inhibition of Rad51 foci observed in p53wt HCT116 cells correlates with our results reporting p53-dependent Rad51 downregulation in this cell line (Fig 2C). The … WebHCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for …

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WebJul 31, 2024 · C HCT116 p53 WT and p53 KO cells were transfected as in (A) and cell cycle analyses were performed 72 h later. Bar chart shows mean values ± SD of three biological replicates ( n = 3). WebMay 1, 2007 · Drug treatment of HCT116 cells triggers immediate stabilization of p53 protein (as evidenced by Western blots) and subsequent suppression or activation of a …

WebDec 2, 2005 · The inhibition of Rad51 foci observed in p53wt HCT116 cells correlates with our results reporting p53-dependent Rad51 downregulation in this cell line (Fig 2C). The specific contribution of p53-dependent mechanisms in repressing HR remains to be established; however, cooperative efficient repression of HR by p53 may be required. WebAug 25, 2009 · The use of p53-specific siRNA in HCT116 (p53 +/+) cells also elicited the same divergent pattern of p53 transcriptional behavior, where Lasp1 mRNA was up …

WebB, p53-KO HCT116 cells were starved overnight and then incubated with vehicle (0.1% DMSO) or aciculatin (10 µM) for different periods. The DNA proportion was subsequently … WebFeb 6, 2004 · Cancer cells with wild-type p53 (HCT116 and RKO) or mutant p53 (MDA-MB-435, SW480, and PC3) were seeded at a low cell density and their ability to form colonies was measured after 5 days of incubation with Nutlin-1. (F) p53 activation by Nutlin-1 does not involve Ser 15 phosphorylation on p53.

WebJan 12, 2024 · Wild-type HCT116 cells (HCT116/p53 +/+) and p53 knockout HCT116 cells (HCT116/p53 −/−) were a gift from Dr. Yao Lin (Fujian Normal University, Fujian, China) …

WebHCT116 p53–/– cells as well as a derivative cell line expressing the p53-Luc fusion were tested and the data presented are an average of three independent experiments. … mini bearing puller toolWebJan 12, 2024 · Wild-type HCT116 cells (HCT116/p53 +/+) and p53 knockout HCT116 cells (HCT116/p53 −/−) were a gift from Dr. Yao Lin (Fujian Normal University, Fujian, China) and originally obtained from Dr Bert Vogelstein (Johns Hopkins University, Baltimore, MD). All human cell lines have been authenticated using STR (or SNP) profiling within the last 3 ... most expensive hotel banffWebOct 21, 2024 · Consistent with the p53-dependent intracellular WNT3 increase, secretion of WNT3 was significantly increased by treatment of 5-FU in p53 +/+ HCT116 cells but not … mini beard trimmer wahl assemblyWebIn p53+/+ cells concurrent G1- and G2-arrests were activated followed by senescence induction. Increased expression of genes associated with senescence, senescence … most expensive hotel in bellagio italyWebApr 1, 2024 · HCT116 p53 +/+ and HCT116-p53 −/− cells were transfected with shRNA to ATM or control shRNA to GFP, and stable cell lines were isolated as described … most expensive hotel in bermudaWebJan 26, 2024 · The key tumor suppressor protein p53, additionally known as p53, represents an attractive target for the development and management of anti-cancer therapies. p53 has been implicated as a tumor suppressor protein that has multiple aspects of biological function comprising energy metabolism, cell cycle arrest, apoptosis, growth and … most expensive hotel in athensWebThe CRC colorectal cancer (CRC) cell lines HCT116 wild-type (wt), HCT116 p53-/-, and HT-29 (mutant; R273H) were employed, covering three different p53 variations. Synergistic … minibear kids camera