How does a bca assay work
WebApr 27, 2024 · The concentration of the target proteins were determined using BCA assay. The purified proteins were stored at -80°C prior to use. SDS-PAGE and Western blotting (WB) ... This work was supported by grants from the National Key R&D Program of China (No.2016YFA0502100 and No. 2016YFC0902001), the key program of the National …
How does a bca assay work
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WebApr 24, 2024 · Answer. While both assays use BCA (bicinchoninic acid) to measure protein concentration of dilute protein samples, the Micro BCA assay is designed for testing … A stock BCA solution contains the following ingredients in a highly alkaline solution with a pH 11.25: bicinchoninic acid, sodium carbonate, sodium bicarbonate, sodium tartrate, and copper(II) sulfate pentahydrate. The BCA assay primarily relies on two reactions. First, the peptide bonds in protein reduce Cu ions from the copper(II) sulfate to Cu (a temperature dependent reaction). The amount of Cu reduce…
WebNov 1, 2024 · Protein quantification by bicinchoninic acid (BCA) assay follows complex kinetics and can be performed at short incubation times. Amongst the available … WebThe BCA Protein Assay combines the protein-induced biuret reaction (see above) with the highly sensitive and selective colorimetric detection of the resulting cuprous cation …
WebOct 2, 2024 · The Standard BCA Protein Assay enables the measurement of protein concentrations from 10 μg/mL to 2000 μg/mL with incubation at 37°C for 30 min or room … WebJun 27, 2024 · Originally developed in 1985, the BCA assay is another colorimetric assay. (3) This two-step assay first makes use of the biuret reaction, in which the protein backbone chelates Cu 2+ ions and reduces them to Cu + ions. In the second step, the Cu + ions react with BCA to form a purple-colored product that absorbs at 562 nm.
WebHow does the BCA assay work? The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent reaction, from the copper solution to Cu+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution.
WebThe formation of the bicinchoninic acid (BCA)–copper complex for the BCA total protein assay. This assay proceeds in two steps: the first being the reduction of Cu 2 + by … pontoonboatsupplyWebCollect the clear supernatant and assay immediately. Cell lysates: Commercial RIPA kits are recommended for preparation of cell lysate samples. For 2 x 106 cells, lyse cells in 0.5 mL RIPA buffer, removing DNA content. Determine the protein concentration using a BCA assay kit (BN01031) with each sample concentration being ≤ 300μg for analysis. pontoon boat sun shadeWebApr 10, 2024 · The aim is to provide a snapshot of some of the most exciting work published in the various research areas of the journal. clear search menu. Journals. Active Journals ... protein quantitation was performed using BCA assay, and they were then boiled. Total cellular proteins were electrophoresed by SDS-PAGE and then probed with the indicated ... pontoon boat sun shadesWebThe BCA Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and primarily relies on two reactions. Firstly, the peptide bonds … pontoonboatsupply.comWebBicinchoninic Acid Assay (BCA) Method. The BCA assay method is based on the fact that the sodium salt of bicinchoninic acid reacts with the cuprous ion generated by the biuret … pontoon boat supply companyWebdifferent proteins in different ways. One may assay the protein of interest in deionized water alone, then in the buffer with possible interfering substances. Comparison of the readings will indicate if an interference exists. Note: Reagents that chelate metal ions, change the pH of the assay, or reduce copper will interfere with the BCA assay. shape fitness chickasha okWebThe BCA protein assay is used for quantitation of total protein in a sample. The principle of this method is that proteins can reduce Cu+2 to Cu+1 in an alkaline solution (the biuret … pontoon boat supplies catalog