Phospho smad
WebNational Center for Biotechnology Information WebPhospho-Smad2 (Ser465/467) (138D4) Rabbit mAb detects endogenous levels of Smad2 only when dually phosphorylated at serines 465 and 467, and may detect Smad3 …
Phospho smad
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WebSmad3 (phospho S423 + S425) was labelled with purified ab52903 at a dilution of 1/100 dilution, while Smad3 was labelled with ab207447 at a … WebZoom Anti-phospho-Smad3 (Ser423/425) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Smad3 (Ser423/425) also known as MAD Mothers against DPP homolog 3 & has been validated in WB. Anti-phospho-Smad3 (Ser423/425) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available …
WebAnti-Smad2 (phospho S255) antibody [EPR2856 (N)] See all Smad2 primary antibodies Description Rabbit monoclonal [EPR2856 (N)] to Smad2 (phospho S255) Host species Rabbit Tested applications Suitable for: Dot blot, IHC-P, IP, WB more details Species reactivity Reacts with: Mouse, Rat, Human Immunogen Synthetic peptide. WebSMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by …
WebThe Phospho-Smad5 (Ser463,Ser465) Antibody has been validated for the following applications: Western Blot. Western Blot analysis of Phospho-Smad5 (Ser463,Ser465) performed on various cell lines using a Phospho-Smad5 (Ser463,Ser465) monoclonal antibody (Product # MA5-35867) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG … WebFeb 2, 2024 · We detected both phospho-SMAD3 and phospho-SMAD2 in AR-negative PC3 cells in the absence of TGF-β treatment but those levels significantly increased after TGF-β treatment (Figure 3A). By contrast, phospho-SMAD3 and phospho-SMAD2 were undetectable in AR-positive Rv1 and C4-2 cells, regardless of TGF-β treatment (Figure 3A …
TGF-B ligands bind receptors consisting of type 1 and type 2 serine/threonine kinases, which serve to propagate the signal intracellularly. Ligand binding stabilizes a receptor complex consisting of two type 1 receptors, and two type 2 receptors. Type 2 receptors then can phosphorylate type 1 receptors at locations on the GS domain, located N-terminally to the type 1 kinase domain. This phosphorylation event activates the type 1 receptors, making them capable of further propagatin…
WebImmunohistochemistry was performed to determine the expression of Smad transducer proteins in NFPAs. Ki-67 was evaluated as an indicator of the proliferative activity of … florida missing money unclaimed fundsWebPhospho-SMAD1 S465 rabbit polyclonal antibody (20 ul) In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol) 2. SMAD1 mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols. florida missing teen foundWebProduct Description. The Phospho-Smad Antibody Sampler Kit contains reagents to investigate the activation of the TGF-β and BMP signaling pathways. The kit contains … great western bank personal loan ratesWebImmunohistochemistry was performed to determine the expression of Smad transducer proteins in NFPAs. Ki-67 was evaluated as an indicator of the proliferative activity of NFPAs. ... Protein levels of Smad3 and phospho-Smad3 (p-Smad3) were significantly lower in patients with invasive NFPAs than in patients with noninvasive NFPAs (P<0.05 and P<0. ... great western bank ppp customer portalWebThe phospho-SMAD3 (Ser423/425) kit enables the cell-based quantitative detection of SMAD3 phosphorylated on Ser423/425, as a readout of the TGFb pathway. ... Overactivation of TGF-ß/SMAD signaling in hepatocytes and HSCs promotes liver fibrosis. Human LX-2* and HepG2 lines were plated (50,000 and 200,000 cells/well respectively) and cultured ... florida mls homes for rentWebRLU = relative light units; p-SMAD2 = phospho-SMAD family member 2. Download Figure Download Powerpoint. Our data suggest that sustained Smad2 phosphorylation in response to TGF-β is dependent on Smad3 activity and is required for myofibroblast differentiation. While acknowledging the role of a canonical immediate Smad2/3-dependent gene ... florida mls map searchWebApr 24, 2008 · The anti-phospho-Smad2 antibody specifically recognized the phosphorylated form of Smad2, whereas the anti-phospho-Smad1/5/8 antibody also reacted to the unphosphorylated form. These antibodies did not react with the other Smads. TGF-β Transforming growth factor-β BSA Bovine serum albumin. Keywords Smad … great western bank ppp